Solution Information | help | |
Enzyme: | cAMP-dependent protein kinase catalytic subunit alpha [Q181K,T183A] | |
inhibitor: | BDBM14029 | |
substrate: | Kemptide | |
Solution Type: | Aqueous | |
pH at Preparation: | 6.8 | |
Temp. Prep.: | 25 | |
Comments: | n/a | |
Solute 1: | lactate dehydrogenase | |
Concentration: | 15 units/mL | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 2: | KCl | |
Concentration: | 100 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 3: | MgCl2 | |
Concentration: | 10 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 4: | beta-mercaptoethanol | |
Concentration: | 1 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 5: | phosphoenolpyruvate | |
Concentration: | 1 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 6: | pyruvate kinase | |
Concentration: | 8 units/mL | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solvent 1: | water | |
Concentration: | 1.0 | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
pH Buffer 1: | MOPS | |
Concentration: | 100 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
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